Oligonucleotide Pool Library Market Size, Share, Growth, and Industry Analysis, By Type (DNA Oligos, RNA Oligos), By Application (Target Capture, CRISPR/Cas9 Designs, Gene Synthesis, Library Preparation, Others), Regional Insights and Forecast From 2025 To 2034

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OLIGONUCLEOTIDE POOL LIBRARY MARKET OVERVIEW

The global oligonucleotide pool library market size is estimated at USD 0.14 billion in 2025 and is expected to reach USD 0.59 billion by 2034 at a CAGR of 17.15% during the forecast period from 2025 to 2034.

The United States Oligonucleotide Pool Library market size is projected at USD 0.05 billion in 2025, the Europe Oligonucleotide Pool Library market size is projected at USD 0.04 billion in 2025, and the China Oligonucleotide Pool Library market size is projected at USD 0.03 billion in 2025.

The chemical synthesis of comparatively tiny segments of nucleic acids with specified chemical structure (sequence) is known as oligonucleotide synthesis. The technology is particularly valuable in modern laboratory practice since it allows for quick and low-cost access to tailored oligonucleotides of the appropriate sequence. It is also beneficial for molecular diagnostics in a variety of disorders, including the identification of infectious diseases such as hepatitis and SARS-COV2, as well as the diagnosis of cancer.

New oligo sequence applications in many domains of biotechnology are expected to drive oligonucleotide pool library market expansion. One of the main developing fields of use for oligonucleotide synthesis is a need of molecular diagnostics within precision medicine.

KEY FINDINGS

COVID-19 IMPACT

Rise in the Health Issues to Advance Market Growth 

The COVID-19 pandemic has been unprecedented and staggering, oligonucleotide pool library experiencing higher-than-anticipated demand across all regions compared to pre-pandemic levels. The sudden rise in CAGR is attributable to the market’s growth and demand returning to pre-pandemic levels.

The COVID-19 pandemic has impacted operations in medical facilities around the world. The sickness has compelled a number of sectors, including various sub-domains of healthcare, to temporarily close their doors. However, there has been a beneficial impact and increase in demand for a variety of healthcare services, particularly oligonucleotide pool library products.

Oligonucleotide pool library products are necessary for the development of test kits, therapies, and vaccinations to combat the new Coronavirus illness. Furthermore, with the rise in coronavirus disease (COVID-19) cases and the need for a viable treatment, fresh types of oligonucleotide therapeutics, comprising mRNA and DNA vaccines, are being developed.

LATEST TRENDS

PCR Primer Category to Stimulate Market Development 

In 2022, the PCR primers category held the largest market share. Primers constitute the most common type of oligonucleotide utilized in PCR. They are part of complementary sequences to the 5' end of the target sequence and are utilized for sequencing or amplification. Primers are increasingly being used in gene sequencing investigations. Because of their usage in fluorescence detection and target amplification, several primer designs with diverse probe- as well as primer-based detection chemicals have been developed.

• In 2024, over 12,000 labs worldwide incorporated oligonucleotide pool libraries in CRISPR and gene-editing workflows, with nearly 45% focused on genome-wide functional screening.

• Approximately 63% of synthetic biology startups used pooled oligo libraries for rapid prototyping, allowing simultaneous testing of 10,000 to 100,000 gene variants per experiment.

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OLIGONUCLEOTIDE POOL LIBRARY MARKET SEGMENTATION

By Type

Based on type; the market is divided into DNA oligos, RNA oligos

DNA oligos is the leading part of the type segment.

By Application

Based on the application; the market is divided into target capture, CRISPR/Cas9 designs, gene synthesis, library preparation, others

Target capture is the leading part of the application segment.

DRIVING FACTORS

Investment by Several Business to Aid Market Expansion

Furthermore, several businesses are making investments in oligonucleotide synthesis that is projected to boost oligonucleotide pool library market share throughout the forecast period. SpeeDx, for example, invested in domestic oligonucleotide manufacture in September 2021 to reduce risk in the supply chain and establish a more crucial sovereign capability for critical diagnostics. Such innovations are projected to expand the use of oligonucleotides in medication development, fueling market growth throughout the forecast period.

High Throughput Sequencing to Foster Industry Progress

The introduction of new techniques for gene expression analysis, including as CRISPR as well as high-throughput sequencing, has eased genetic screening and genome editing. Such advancements have been seen to have an impact on the requirement for oligonucleotide synthesis. Manufacturers invest in technology development to enable the production of lengthy Oligonucleotides with greater precision and yield to meet the growing demand for custom oligos.

• More than 210 institutions globally integrated oligonucleotide libraries into cancer and rare disease studies, enabling analysis of over 50,000 unique DNA sequences per trial.

• In 2024, pooled libraries supported target screening across 28 major pharmaceutical pipelines, with each pool comprising over 20,000 custom-designed oligos.

RESTRAINING FACTORS

Difficult Delivery of the Product to Limit the Market Evolution  

On the contrary, the complexity connected with therapeutic oligos and the difficult delivery of oligonucleotide medications to specific targets may stymie the oligonucleotide pool library market growth expansion.

• Researchers report that approximately 31% of projects using large oligo pools encounter delays due to computational bottlenecks in handling datasets exceeding 500 GB.

• The cost per base for high-fidelity oligo synthesis can reach USD 0.15, making larger pools with 100,000+ sequences financially challenging for smaller research teams.

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OLIGONUCLEOTIDE POOL LIBRARY MARKET REGIONAL INSIGHTS

Constantly Developing Medical Facilities in Asia Pacific to Support Market Advancement

Due to constantly developing medical facilities, economic development, and increased government backing and investment, Asia Pacific is predicted to demonstrate the quickest market growth throughout the projection period. Furthermore, an increase in incidence of chronic conditions such as COVID-19, cardiovascular disease, cancer, and others, as well as an expansion in government support and expenditure for improving people's health, stimulates market growth.

Furthermore, key competitors in this expanding region provide a profitable opportunity for the oligonucleotide synthesis market. For example, in 2020, IDT (US) established IDT China as a legitimate corporate entity in China in order to better serve its existing clientele in the region, reach out to new clients, and provide simpler access to its whole array of industry-leading products, such as bespoke RNA and DNA oligos, probes, and assays.

KEY INDUSTRY PLAYERS

Key Players Focus on Partnerships to Gain a Competitive Advantage

Prominent market players are making collaborative efforts by partnering with other companies to stay ahead of the competition. Many companies are also investing in new product launches to expand their product portfolio. Mergers and acquisitions are also among the key strategies used by players to expand their product portfolios.

The analyst provides a comprehensive picture of the market through the study, synthesis, and summarization of data from various sources, as well as an examination of important variables such as financial gain, sales prices, competition, and promotions. It identifies the key industry influencers and presents various market facets. The information provided is thorough, reliable, and the result of extensive primary and secondary research. The market report provides a comprehensive competitive landscape as well as an in-depth vendor evaluation methodology and analysis based on both qualitative and quantitative studies to accurately forecast market growth.

The reports cover important advances with in market, as well as inorganic and organic growth strategies. Various companies are concentrating on organic business expansion such as product announcements, product approvals, and other things like patents and events. Acquisitions, partnerships, and collaborations were among the inorganic growth strategies observed in the market. The above activities have paved the way for market participants to expand their business and customer base. With the growing demands for filter products in the international market, market participants in the market are expected to benefit from substantial growth opportunities in the near future.

• Twist Bioscience: Twist Bioscience synthesized over 1.4 billion bases of DNA in oligo pool format in 2024, supporting more than 1,800 clients in synthetic biology, immunology, and diagnostics.

• Daicel Arbor Biosciences: Daicel Arbor Biosciences delivered oligo libraries to over 950 global research centers, with catalog and custom pools exceeding 2.5 million unique oligonucleotide sequences in 2024 alone.

List of Top Oligonucleotide Pool Library Companies

• Twist Bioscience
• Daicel Arbor Biosciences
• Synbio Technologies
• Dynegene Technologies
• General Biosystems
• Integrated DNA Technologies
• Agilent
• LC Sciences
• CustomArray
• Creative Biogene

REPORT COVERAGE

This research offers a thorough analysis of the global market, covering all relevant areas. This includes everything from a broad market overview to micro-level information about size of the market, degree of competition, evolution trend, opportunities in the market, important market drivers, and SWOT opportunities and threats. The reader can use the study to influence industry competitiveness and competitive environment strategies to increase potential profit. Additionally, it offers a straightforward framework for assessing and gaining access to the situation of the corporate organization.

In a nutshell, this study should be read by all market participants, traders, academics, analysts, business planners, and anyone else with an interest in the market. The market competitive landscape is another area that the report structure focuses on. This report presents in depth information the share of the market, macroeconomic indicators, product scenario, operation circumstances of the major players, which aids readers in the industry in recognizing the key rivals and better understanding the market's competitive landscape.